Mouse Fatty Acid Binding Protein 5 Epidermal ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Fatty Acid Binding Protein 5, Epidermal in biofluid samples, such as tissue homogenates, breast milk and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . FABP5 present in samples reacts with an anti-Mouse FABP5 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-FABP5 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active FABP5 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse FABP5. Detection Target: Mouse Fatty Acid Binding Protein 5, Epidermal Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.054ng/mL Storage Conditions: Store all components at 4┬░C This Mouse FABP5 activity assay is for the quantitative determination of Fatty Acid Binding Protein 5, Epidermal in samples including tissue homogenates, breast milk and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.